|
KMID : 1007519990080020134
|
|
Food Science and Biotechnology
1999 Volume.8 No. 2 p.134 ~ p.138
|
|
|
Simultaneous Determination of Pyruvate , Cholesterol , and Ethanol using a Multiple Cathode Oxygen Electrode System
|
|
Kim, Tai Jin
Noh, Bong Soo/Kim, Jung Ho
|
|
|
Abstract
|
|
|
|
A multiple cathode system was prepared with an oxygen electrode having one anode and multiple cathodes. Three components were simultaneously determined. by five immobilized enzymes with the multiple cathode system. Pyruvate oxidase, cholesterol oxidase and cholesterol esterase, alcohol oxidase, and catalase were respectively immobilized for the determination of pyruvate, cholesterol, and ethanol by the multiple biosensor system. The concentrations of components determined by the enzyme sensors were based on the maximum slope of oxygen consumption from the enzyme reaction. The response time for each analysis was about l min. The optimum condition for simultaneous determination was found to be 0.1 M potassium phosphate buffer, pH 7.0 at 35℃. Effects of various interfering compounds such as sugars and organic acids on this system were investigated. Less than 8% of error was found in the determination of pyruvate. This system was confirmed by conventional methods. It was concluded that this study could be used for an efficient method to determine pyruvate, cholesterol, and alcohol in foods, simultaneously.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|